For when part of a character emits light without an energy or heat source, such as glowing in the dark.
| Name | Series | Gender | Rank | ||
|---|---|---|---|---|---|
| 1 |  | Bill Cipher | Gravity Falls | Male | 752 |
| 2 |  | Midna | The Legend of Zelda: Twilight Princess | Female | 1,134 |
| 3 |  | All For One | Boku no Hero Academia | Male | 2,894 |
| 4 |  | King Minos | ULTRAKILL | Male | 3,486 |
| 5 |  | Ori | Ori and the Blind Forest | Other | 4,148 |
| 6 |  | Mareep | Pokédex | Other | 6,235 |
| 7 |  | Aurelion Sol | League of Legends | Male | 6,471 |
| 8 |  | Navi | The Legend of Zelda: Ocarina of Time | Female | 6,820 |
| 9 |  | Zero | The Nightmare Before Christmas | Male | 8,554 |
| 10 |  | King Sisyphus | ULTRAKILL | Male | 9,907 |
| 11 |  | Voyager | Fate/Requiem | Male | 10,884 |
| 12 |  | White Lady | Hollow Knight | Female | 12,665 |
| 13 |  | Lamia | Punishing: Gray Raven | Female | 13,127 |
| 14 |  | Zant | The Legend of Zelda: Twilight Princess | Male | 14,220 |
| 15 |  | Xurkitree | Pokédex | Other | 14,565 |
| 16 |  | Líf | Fire Emblem Heroes | Male | 16,428 |
| 17 |  | Loop | In Stars and Time | Other | 16,958 |
| 18 |  | C.Q. Cumber | Splatoon 2 | Male | 17,458 |
| 19 |  | Shyren | UNDERTALE | Female | 17,751 |
| 20 |  | Orochi | The King of Fighters '97 | Other | 17,849 |
